PhD position BioMolecular Analysis

VU University Amsterdam (VU) - PhD position BioMolecular Analysis

Project: ‘Innovative Analytical Methodologies for Biopharmaceuticals’

Job description

The PhD project, we address the specific situation that unknown, bioactive proteins are present next to known compounds, examples being peptide or protein libraries, or in-vitro or in-vivo samples containing the parent product and potentially bioactive degradation or biotransformation products. In both cases, the need to efficiently discover and characterize unknown bioactive proteins at low concentrations in complex matrices puts special demands on the analytical techniques to be used. Our strategy is to integrate three key elements in one measurement: (1) separation techniques such as size-exclusion or ion-exchange chromatography that maintain the biological activity of proteins during the separation process (2) UV and mass spectrometric detection to reveal the chemical structure of the proteins/peptides eluting from the separation column, and (3) on-line or at-line biochemical assays reflecting the biological activity of proteins. The research is done in close collaboration between the Divisions of BioMolecular Analysis and Target and System Biochemistry/Medicinal Chemistry at the VU University.

Requirements

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